by Shaikh
(Albany, NY, USA)
Original Text: ISTD
Internal Standard method (ISM) is a useful tool to rectify the effect of varying analytical conditions during single analysis run. This study is a simple and inexpensive experimental approach for understanding the scope and use of ISM. Experiment is designed for graduate or undergraduate students of analytical chemistry and even for other disciplines of chemistry. This experiment takes less time and space. Hence giving more chance for students to participate by themselves using less equipment. Criteria for selection of internal standard (ISTD) are discussed and most of them are experimentally demonstrated. Spectrophotometry is used as analytical tool. Several compounds (like K2Cr2O7, NiSO4, CuSO4, KNO3) are examined (by obtaining 200-800nm wavelength spectra) for selection of a pair of compound that can be used as internal standard for each other. Out of these NiSO4 and KNO3 are selected. NiSO4 is treated as internal standard and KNO3 as analyte. Experimental condition is changed deliberately for one solution in order to demonstrate the effectiveness and usefulness of ISM. Single and multiple standard(s) calibrations are carried out with and without using ISTD. Correlation coefficient values of data using ISTD found significantly good, that ensures the quality results under varying experimental conditions.
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Revised Text:
The Internal Standard Method (ISM) is a useful tool for rectifying the effects of varying analytical conditions during single analysis runs. This study shows a simple and inexpensive experimental approach for understanding the scope and use of ISM.
The experiment is designed for graduate or undergraduate students of analytical chemistry and even for other disciplines of chemistry. This experiment takes less time and space, giving more chances for students to participate by themselves using less equipment. Criteria for selection of Internal Standards (ISTD) are discussed and most of them are experimentally demonstrated.
Spectrophotometry is used as an analytical tool. Several compounds (like K2Cr2O7, NiSO4, CuSO4, KNO3) are examined (by obtaining 200-800nm wavelength spectra) for selection of a pair of compounds that can be used as internal standards for each other. Out of these, NiSO4 and KNO3 are selected. NiSO4 is treated as the internal standard and KNO3 as analyte.
Experimental conditions are changed deliberately for one solution in order to demonstrate the effectiveness and usefulness of ISM. Single and multiple standard(s) calibrations are carried out with and without using ISTD.
Correlation coefficient values of data using ISTD were found to be significantly good. That ensures the quality results under varying experimental conditions.
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