JPR

by Sheeba
(Chennai, Tamil Nadu, India)

Original Text: JPR


MicroRNAs (miRNAs) are a class of small, non coding RNAs that regulate post-transcriptional expression in plants and animals. A total of 8433 miRNAs has been identified from various crop plants such as rice and wheat. Among the priority species, banana (Musa spp.) is a model system for herbaceous flowering plants in which characteristics of miRNAs are poorly understood. In this study, computational prediction of miRNAs from banana EST and BAC sequences was performed and resulted in the finding of 18 banana miRNAs belonging to 16 miRNA families. For the 18 miRNAs, 25 banana ESTs were found as their potential targets. Most of these targets encode transcriptional factors which play a role in banana development. For experiment validation, four out of 18 miRNA representatives were cloned and sequenced. This is the first report to predict and identify miRNAs in banana.

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Revised Text:

Micro-RNAs (miRNAs) are a class of small, non-coding RNAs that regulate post-transcriptional expression in plants and animals. A total of 8433 miRNAs have been identified from various crop plants, such as rice and wheat. Among the priority species, banana (Musa spp.) is a model system for herbaceous flowering plants in which characteristics of miRNAs are poorly understood.

In this study, computational prediction of miRNAs from banana EST and BAC sequences was performed, and resulted in the finding of 18 banana miRNAs belonging to 16 miRNA families. For the 18 miRNAs, 25 banana ESTs were found as their potential targets. Most of these targets encode transcriptional factors which play a role in banana development. For experiment validation, four out of the 18 miRNA representatives were cloned and sequenced. This is the first report to predict and identify miRNAs in banana.

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